Whereas the vulva of wild-type worms developed into the pyramidal stage (81 of 82 worms), the P6.p of mir-71(n4115, lf) mutant worms divided only once (83 of 89 worms). The computation-based prediction that age-1 and pdk-1 are potential targets of miR-71 was also reported in a recent study focusing on miRNA functions in aging where the mRNA level of pdk-1 was shown to be up-regulated in mir-71 worms (14). (C) Fluorescence and differential interference contrast (DIC) images showing that the age-1 3′UTR reporter was repressed in mir-71(+) worms (3/4 transgenic lines) but not in mir-71(lf) worms (4/4 transgenic lines). The transcript level of unc-31 was increased in mir-71(lf) worms, compared with that of wild-type controls that were normalized to the value of 1. MiR-71 represses the expression of age-1 and unc-31 through the actions on their 3′UTR, but miR-71 is not required for arresting M cell division during L1 diapause.

miR-71 Is Not Required for Arresting Seam Cell or M-Cell Divisions During L1 Diapause.

Be sure to enable third-party account backup and restore if you use Duo Mobile to generate passcodes for logging into applications like Instagram, Facebook, Snapchat, or other web services. To compare the survival rates between strains, we simulated the survival rate of each genotype to 100 arbitrary “individual worms” and performed the log-rank test in Graphpad Prism 4. This result suggests that the high expression of miR-71 during L1 diapause is induced or maintained by other signaling pathways. We asked whether the expression of miR-71 was regulated by DAF-16, which is required during L1 diapause for long-term survival (2). It is possible that other miRNAs, including those in the let-7 family, control developmental timing in other tissues during the recovery phase after L1 starvation.

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